Immunoproteomic analysis of the antibody response obtained in Nile tilapia following vaccination with a Streptococcus iniae vaccine.

Development and efficacy of a novobiocin-resistant Streptococcus iniae as a novel vaccine in Nile tilapia (Oreochromis niloticus).

A novel attenuated Streptococcus iniae vaccine was developed from a virulent strain of Streptococcus iniae (ISET0901) through selection for novobiocin resistance (named ISNO). The safety of ISNO was then evaluated in Nile tilapia (Oreochromis niloticus) through intraperitoneal (IP) injection. When male tilapia (average weight 10 g) were IP injected with 2×10(7) colony-forming units (CFU) of the...

Serum antibody response of rainbow trout, Oncorhynchus mykiss to two species of pathogenic bacteria; Streptococcus iniae and Lactococcus garviae

One of the most important bacterial diseases in aquaculture is Streptococosis/loctococosis. Meanwhile this disease has been known as a zoonosis disease, and then fish vaccination against this disease is important. In this study the specific immunological responses of rainbow trout (Oncorhynchus mykiss) to vaccination with bivalent lactococcal/streptococcal vaccine were investigated. Two hundred...

Effect of parasitism on vaccine efficacy against Streptococcus iniae in Nile tilapia

Vaccination of rainbow trout against Streptococcus iniae infection: comparison of different routes of administration and different vaccines

Antibody production and clinical efficacy (relative percent survival RPS) were measured in 40±5g rainbow trout after immunization with two types of Streptococcus iniae vaccines consisting of formalin killed cells (FKC) and FKC enriched with the bacterial extracellular products (ECP) administered by intraperitoneal (i.p), immersion and oral routes at 16±1°C for 18 weeks. No significant differenc...

Efficacy of a Streptococcus iniae modified bacterin delivered using OraljectTM technology in Nile tilapia (Oreochromis niloticus)

This study evaluated the potential to orally deliver a previously developed Streptococcus iniae vaccine in tilapia using OraljectTM technology. This technology was developed to administer bioactive compounds to monogastric animals, and has been shown to be effective for delivery of a variety of antigens in numerous fish species. Two different formulations containing two doses of vaccine (four t...